PLANT RESPONSE TO BIOSTIMULANTS BASED ON NATURAL MATERIAL

Soil is a complex environment inhabited by varied soil microbial communities interacting with each other solubilizing and mineralizing organic matter and different chemical compound secreted into the soil and making it available to the plants. Beneficial microbial interactions with roots influence plant mineral nutrient availability.

A plant biostimulant is a substance or microorganisms when applied to plants enhance nutrition efficiency and tolerance to abiotic stress regardless of its nutrients content (du Jardin 2015). The proposed definition is “Plant biostimulants are substances and materials, with the exception of nutrients and pesticides which when applied to plant, seeds or growing substrates in specific formulations, have the capacity to modify physiological processes of plants in a way that provides potential benefits to growth, development and/or stress response” (du Jardin 2012). Biostimulant can also be defined as “A formulated product that improves plant productivity by a mechanism of action that is not the sole consequence of the presence of known essential plant nutrients, plant hormones, plant growth regulators, or plant protective compounds” (Yakhin et al., 2017). Biostimulants are not fertilizers, they do not contain nutrient intended to be delivered to plants. The first in-depth analysis of plant biostimulant with emphasis on biostimulant systemization and categorization was made on the basis of biochemical, physiological function, mode of action and origin by du Jardin (2012, 2015).

Many biostimulants improve photosynthetic efficiency and plant defense against pathogens. Plant growth-promoting rhizobacteria (PGPR) can be effective in biostimulation of plant growth (Calvo et al., 2014). The dual function of microorganisms (biostimulant and biocontrol) can be seen when applied to plants. There are eight categories of biostimulants (du Jardin 2012):

  1. Humic substances
  2. Complex organic materials
  3. Beneficial chemical elements
  4. Inorganic salts (such as phosphite)
  5. Sea weed extracts
  6. Chitin and chitosan derivatives
  7. Antitranspirants
  8. Free amino acids and other N-containing substances.

The subcategories proposed by the Biostimulant Coalition are: antioxidants, amino acids materials, biomolecule, enzymatic extracts, fulvic acid materials, humic acid materials, microbial inoculants, microbial soil amendments, mycorrhizal fungi, PGPRs, phytohormones, seaweed extract materials (du Jardin 2015).

On the basis of their source and content, biostimulants are classified into three major groups (Kauffman et al., 2007). These group are humic substances, hormone containing products (HCP) and amino acid containing group.

 Humic substances (HS):

Humic substances (HS) are natural constituents of the soil organic matter, resulting from the decomposition of plant, animal and microbial residues, as well as from the metabolic activity of soil microbes using these as substrates. Humic substances exhibit stimulatory effect on plant growth. It improves soil fertility by improving soil structure and porosity through soil aggregation (Bronick and Lal 2005), increase water holding capacity and bioavailability of nutrient.  HS are collection of heterogeneous compounds, categorized according to their molecular weights and solubility into humins, humic acids and fulvic acids (du Jardin 2015). Low molecular size fraction reaches the plasmalemma of higher plant cells and is taken up by the plants while high molecular size fraction is not absorbed but only interacts with cell wall.  Humic matter displays a hormone like activity. It is not clear whether the hormonal activity is linked to the chemical structure of HS (functional group of humic molecule) or it depends on hormones of microbial origin entrapped into them (Nardi et al., 2002).

Hormone containing products (HCP):

Hormone containing products such as seaweed extracts, contain identifiable amounts of active plant growth substances such as auxins, cytokinins, or their derivatives (Kauffman et al., 2007). Seaweeds include green, brown and red macroalgae. Liquid extract of seaweed when applied to seeds or added to soil stimulate plant growth. Extract derived from Polysiphonia, Ulva and Cladophora can be nontoxic biostimulants of plant growth Godlewska et al. (2016).

Extracts derived from algae contain:

  • Polysaccharides such as galactan, fucoidan, alginate, and laminarin
  • Proteins example lectins
  • Polyunsaturated fatty acids (PUFAs)
  • Pigments such as chlorophylls, carotenoids, and phycobiliproteins
  • Polyphenols example phenolic acids, flavonoids, cinnamic acid, isoflavones, benzoic acid, quercetin and lignans
  • Minerals such as potassium, magnesium, calcium and sodium
  • Plant growth hormones such as cytokinins, auxins, gibberellins, and abscisic acid (Chojnacka 2012).

Seaweed extracts act as biostimulants, enhancing seed germination and establishment, improving plant growth, yield, flower set and fruit production, increasing resistance to biotic and abiotic stresses, and improving postharvest shelf life ( Rayorath et al., 2008;  Khan et al., 2009; Craigie 2011).

Amino acid containing products (AACP):

Protein based products can be divided into two categories:

i  Protein hydrolysate: Protein hydrolysate formulation can be used as a biostimulant to improve crop productivity. It consists of a mixture of peptides and amino acids of animals and plant origin. Protein hydrolysates can improve crop tolerance to abiotic stresses (Ertani et al., 2013).

ii Individual amino acids (glutamate, glutamine, proline and glycine betaine):  Amino acid can easily be absorbed by plant root and through foliage (Nacry et. al.,2013 and Stiegler et al., 2013).

Application of plant derived protein hydrolysate containing amino acids and small peptides elicited a hormone like activity and  enhanced nitrogen uptake (Colla et al., 2014). Amino acid and peptide mixtures are obtained by chemical and enzymatic hydrolysis from plant sources (crop residue) and animal wastes (e.g. collagen, epithelial tissues) (du Jardin 2012 and Calvo et al., 2014). Protein hydrolysates and specific amino acids including proline, betaine, their derivatives induce plant defense responses and increase plant tolerance to abiotic stresses (salinity, drought, temperature etc.) and oxidative conditions (Ashraf and Foolad 2007; Chen and Murata 2008)

Amendment of organic matter into the soil is beneficial, it conserve nutrients, reduce environmental pollution and improves soil physico-chemical structure.  Organic farming promote the use of biocontrol agent and biofertilizer. The biostimulants may be used to attain better germination, improved growth and yield and can be understood only when the interaction between plant, biostimulant and environment is addressed.

References:

Ashraf, M. and Foolad, M. R.  2007 Roles of Glycine Betaine and Proline in Improving Plant Abiotic Stress Resistance. Environ Exp. Bot. 59: 206–216

Bronick, C. J. and Lal, R. 2005 Soil Structure and Management: A Review. Geoderma 124: 3-22

Calvo, P., Nelson, L. and Kloepper, J. W. 2014 Agricultural Uses of Plant Biostimulants. Plant Soil 383: 3-41

doi 10.1007/s11104-014-2131-8

Chen, T. H. H. and Murata, N. 2008 Glycinebetaine: An Effective Protectant Against Abiotic Stress in Plants. Trends Plant Sci. 13: 499–505

Chojnacka, K., Saeid, A., Witkowska, Z. and Tuhy, Ł. 2012 Biologically Active Compounds in Seaweed Extracts—The Prospects for the Application.  The Open Conference Proceedings Journal 3(1): 20–28

Colla, G., Rouphael, Y., Canaguier, R., Svecova, E.  and Cardarelli, M. 2014 Biostimulant Action of a Plant-Derived Protein Hydrolysate Produced Through Enzymatic Hydrolysis.  Front Plant Sci. 5: 448

doi:  10.3389/fpls.2014.00448

Craigie, J. S. 2011 Seaweed Extract Stimuli in Plant Science and Agriculture J. Appl. Phycol.  23 (3): 371- 393

 doi:10.1007/s10811-010-9560-4

du Jardin, P. 2012 The Science of Plant Biostimulants – A Bibliographic Analysis pp 1-37

Plant_Biostimulants_final_report_bio_2012_en.pdf

du Jardin, P. 2015 Plant biostimulants: Definition, Concept, Main Categories and Regulation. Scientia Horticulturae 196:  3-14

https://doi.org/10.1016/j.scienta.2015.09.021

Ertani, A., Schiavon, M., Muscolo, A. and Nardi, S. 2013 Alfalfa Plant-Derived Biostimulant Stimulate Short-Term Growth of Salt Stressed Zea Mays L. Plants. Plant Soil 364 (1-2): 145–158

10.1007/s11104-012-1335

Godlewska, K.,   Michalak, I., Tuhy, Ł.  and Chojnacka, K. 2016 Plant Growth Biostimulants Based on Different Methods of Seaweed Extraction with Water. Hindawi Publishing Corporation.  BioMed Research International volume 2016 Article ID 5973760   11 pages

http://dx.doi.org/10.1155/2016/5973760

Kauffman, G. L., Kneivel, D. P. and Watschke, T. L. 2007 Effects of a Biostimulant on the Heat Tolerance Associated with Photosynthetic Capacity, Membrane Thermostability and Polyphenol Production of Perennial Ryegrass. Crop Sci. 47: 261-267

 Khan, W., Rayirath, U.P., Subramanian, S., Jithesh M. N., Rayorath, P., Hodges D. M., Critchley, A. T., Craigie, J. S., Norrie, J. and Prithiviraj, B. 2009  Seaweed Extracts as Biostimulants of Plant Growth and Development  J. Plant Growth Regul.   28 (4): 386 – 399

 doi:10.1007/s00344-009-9103-x

Nardi, S., Pizzeghelloa, D., Muscolob, A. and Vianelloc, A. 2002 Physiological Effects of Humic Substances on Higher Plants. Soil Biol. & Biochem. 34: 1527–1536

Rayorath, P., Jithesh, M. N., Farid, A., Khan, W., Palanisamy, R., Hankins, S. D., Critchley, A.T. and Prithviraj, B. 2008 Rapid Bioassays to Evaluate the Plant Growth Promoting Activity of Ascophyllum Nodosum (L.) Le Jol. Using a Model Plant, Arabidopsis thaliana (L.) Heynh.  J.  Appl.  Phycol.  20 (4): 423- 429

doi:10.1007/s10811-007-9280-6

Yakhin, O. I., Lubyanov, A. A., Yakhin, I. A. and Brown, P. H. 2017 Biostimulants in Plant Science: A Global Perspective.  Front Plant Sci. 7: 2049

doi:  10.3389/fpls.2016.02049

Nacry, P., Bouguyon, E. and Gojon, A. 2013 Nitrogen Acquisition by Roots: Physiological and Developmental Mechanisms Ensuring Plant Adaptation to a Fluctuating Resource. Plant Soil 370: 1-29

 Stiegler, J. C., Richardson, M. D., Karcher, D. E., Roberts, T. L. and Norman, R. J. 2013 Foliar Absorption of Various Inorganic and Organic Nitrogen Sources by Creeping Bentgrass. Crop Sci. 52: 1148-1152

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